Nova TSA mIHC kit dye composition:
|
PLUS kit |
2-color |
3-color |
4-color |
5-color |
6-color |
7-color |
8-color |
9-color |
Excitation |
Emission |
Similar fluorescent dyes |
|
DAPI |
√ |
√ |
√ |
√ |
√ |
√ |
√ |
√ |
350 |
420 |
DAPI |
|
TYR-480 |
|
|
|
|
√ |
√ |
√ |
√ |
450 |
480 |
Opal480 |
|
TYR-520 |
|
√ |
√ |
√ |
√ |
√ |
√ |
√ |
490 |
520 |
FITC/AF488/Opal520/cy2 |
|
TYR-540 |
|
|
|
|
|
|
|
√ |
515 |
540 |
AF514/Opal540 |
|
TYR-570 |
|
√ |
√ |
√ |
√ |
√ |
√ |
√ |
550 |
570 |
CY3/AF555/Opal570、PE |
|
TYR-620 |
|
|
|
√ |
√ |
√ |
√ |
√ |
590 |
620 |
AF594/Opal620 |
|
TYR-650 |
|
|
|
|
|
|
√ |
√ |
630 |
650 |
CY5/Opal650 |
|
TYR-690 |
|
|
√ |
√ |
√ |
√ |
|
|
640 |
690 |
AF647/Opal690 |
|
TYR-700 |
|
|
|
|
|
|
√ |
√ |
680 |
700 |
CY5.5/AF680 |
|
TYR-780 |
|
|
|
|
|
√ |
√ |
√ |
750 |
780 |
CY7/Opal780 |
Common confocal microscope parameters and their correspondence to Neorise Nova dyes:
|
Common focus parameters |
Neorise Nova dyes |
EX excitation wavelength |
EM emission wavelength |
|
FITC/488 |
520 |
490 |
520 |
|
549 |
570 |
550 |
570 |
|
594 |
620 |
590 |
620 |
|
633/647 |
690 |
630 |
690 |
Note: The parameter Settings of confocal microscopes vary from model to model.
When recommending kits to customers, please ask customers to provide the excitation and emission wavelength parameters of their equipment.
